#10244-001	0.5 ml
#10244-005	5 × 0.5 ml

OriBio^TM 2× Pfu PCR Mix with loading dye is supplied as a 2× concentration mixture containing a blend of Pfu DNA Polymerase, dNTPs, Mg²⁺, reaction buffer and stabilizer. It is generally used at 1× concentration with DNA template and primers in a total reaction volume of 20 or 50 μl. The fidelity of Pfu DNA polymerase is approximately 4 to 6 times that of Taq DNA polymerase. It is suitable for amplification of DNA fragments up to 10 kb in length with relatively higher fidelity than using Taq DNA polymerase alone. Its benefits include quick and easy setup, high sensitivity, specificity, and good stability, minimizing manual error, time saving, contamination reduction, and is ideal for highthroughput gene detection and rapid clone screening. 2× Pfu PCR Mix generates blunt-end PCR products, which cannot be used directly for a TA cloning reaction. The dye-containing end products after PCR reaction can be loaded directly for gel electrophoresis and detection, and can be purified for subsequent experiments. The red dye contained migrates at a rate which is close to that of a double stranded DNA fragment of 300 bp in a 1% TAE agarose gel.

High fidelity PCR
Generation of PCR products for cloning and expression
RT-PCR for cDNA cloning and expression
Blunt-end PCR cloning

@ -20°C for 1 year. Avoid repeated freeze and thaw cycles.

• 6 times higher fidelity than Taq DNA Polymerase
• Amplification of PCR products up to 10 kb
• Blunt-end PCR products
• Ease of use with minimal steps
• Time-saving and less error
• Analyzing PCR products without additional steps

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