Pfu DNA Polymerase
| #10242-001 | 250 U | |
| #10242-005 | 5 × 250 U |
OriBioTM Pfu DNA Polymerase is a Pyrococcus furiosis clone of DNA polymerase gene expressed in E.coli . The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5´→3´ direction. Pfu DNA Polymerase also exhibits 3´→5´ exonuclease (proofreading) activitythat enables the polymerase to correct nucleotide incorporation errors. It has no 5´→3´ exonucleaseactivity. Pfu DNA polymerase is 6 times more accurate than Taq DNA polymerase. The enzymeis highly thermostable (90% active after 1 hour @ 95℃), and has the capability of incorporatingmodified nucleotides. Pfu DNA polymerase generates blunt-end PCR products, which cannot be used directly for a TA cloning reaction.
High fidelity PCR
Generation of PCR products for cloning and expression
RT-PCR for cDNA cloning and expression
Blunt-end PCR cloning
Generation of PCR products for cloning and expression
RT-PCR for cDNA cloning and expression
Blunt-end PCR cloning
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|
10242-001 |
10242-005 |
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Pfu DNA Polymerase (2.5 U/μl) |
100 μl |
5 × 100 μl |
|
10× Pfu PCR Buffer |
1 ml |
5 × 1 ml |
@ -20°C for 1 year. Avoid repeated freeze and thaw cycles.
• 6 times higher fidelity than Taq DNA Polymerase
• Amplification of PCR products up to 10 kb
• Blunt-end PCR products
• Amplification of PCR products up to 10 kb
• Blunt-end PCR products