2× HiFiTaq PCR Mix (Loading Dye-Free)
| #10236-001 | 0.5 ml | |
| #10236-005 | 5 × 0.5 ml |
OriBio™ 2× HiFiTaq PCR Mix (w/o loading dye) is supplied as a 2× concentration mixture containing a blend of HiFiTaq DNA Polymerase, dNTPs, Mg2+, reaction buffer and stabilizer. It is generally used at 1× concentration with DNA template and primers in a total reaction volume of 20 or 50 μl. The fidelity of HiFiTaq DNA polymerase (a mixture of recombinant Taq DNA polymerase and a high fidelity proofreading DNA polymerase of choice) is approximately 4 to 6 times that of the Taq DNA polymerase. It is suitable for amplification of DNA fragments up to 10 kb in length with relatively higher fidelity than using Taq DNA polymerase alone. Its benefits include quick and easy setup, high sensitivity, specificity, and good stability, minimizing manual error, time saving, contamination reduction, and is ideal for high-throughput gene detection and rapid clone screening. The PCR products generated using 2× HiFiTaq PCR Mix contain dA overhangs at the 3´end, and are suitable for downstream TA cloning.
Higher-fidelity PCR of < 10 kb fragment
High-throughput PCR
Site-directed Mutagenesis
High-throughput PCR
Site-directed Mutagenesis
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10236-001 |
10236-005 |
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2× HiFiTaq PCR Mix (Loading Dye-Free) |
0.5 ml |
5 × 0.5 ml |
|
ddH2O |
0.5 ml |
5 × 0.5 ml |
@ -20°C for 1 year. Avoid repeated freeze and thaw cycles.
• 6 times higher fidelity than Taq DNA Polymerase
• Amplification of PCR products up to 10 kb
• Great sensitivity with high yield
• Ease of use with minimal steps
• Time-saving and less error
• Amplification of PCR products up to 10 kb
• Great sensitivity with high yield
• Ease of use with minimal steps
• Time-saving and less error