ORITaq DNA Polymerase
#10222-001 |
500 U |
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#10222-005 |
5 × 500 U |
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ORITaq DNA Polymerase is purified from E. coli expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has a 5´→3´ DNA polymerase and a 5´→3´ exonuclease activity but lacks a 3´→5´ exonuclease activity. Taq DNA polymerase is heat-stable and synthesizes DNA at elevated temperatures from single-stranded templates at the presence of primers. PCR products generated using ORITaq DNA Polymerase contain 3’dA overhangs, and can be used for downstream TA cloning.
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10222-001
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10222-005
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ORITaq DNA Polymerase (5 U/μl)
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100 μl
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5 × 100 μl
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10× ORITaq PCR Buffer*
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1 ml
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5 × 1 ml
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*10× ORITaq PCR Buffer : 100 mM Tris-Cl (pH 8.3), 15 mM MgCl2, 500 mM KCl.
@ -20°C for 2 years. Avoid repeated freeze and thaw cycles.
• High purity recombinant Taq Polymerase
• Amplification of PCR products up to 5 kb
• Add Mg2+ in buffer for direct use
• Good sensitivity with high yield in amplification