#10220-001	500 U
#10220-005	5 × 500 U

ORITaq DNA Polymerase is purified from E. coli expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has a 5´→3´ DNA polymerase and a 5´→3´ exonuclease activity but lacks a 3´→5´ exonuclease activity. Taq DNA polymerase is heat-stable and synthesizes DNA at elevated temperatures from single-stranded templates at the presence of a primer. PCR products generated using ORITaq DNA Polymerase contain 3’dA overhangs, and can be used for downstream TA cloning. PCR buffer with this product does not contain Mg²⁺; a tube of MgCl_² is provided separately.

	10220-001	10220-005
ORITaq DNA Polymerase (5 U/μl)	100 μl	5 × 100 μl
10× Taq PCR Buffer (Mg2+-free)*	1 ml	5 × 1 ml
25 mM MgCl2	200 μl	1 ml

*10× ORITaq PCR Buffer(Mg²⁺-free) : 100 mM Tris-Cl (pH 8.3), 500 mM KCl.

@ -20°C for 2 years. Avoid repeated freeze and thaw cycles.

• High purity recombinant Taq Polymerase
• Amplification of PCR products up to 5 kb
• Good sensitivity with high yield in amplification
• Buffer without Mg²⁺ allows reaction optimization

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