HiFiTaq DNA Polymerase
| #10232-001 | 500 U | |
| #10232-005 | 5 × 500 U |
OriBioTM HiFiTaq DNA Polymerase is an enzyme mixture composed of optimized ratio of recombinant Taq DNA polymerase with a high fidelity proofreading polymerase of choice. Mixing the proofreading enzyme with Taq DNA polymerase increases fidelity approximately four to six times more than that of Taq DNA polymerase alone, and it allows amplification of simple and complex DNA templates over a broad range of target sizes. Amplicons of up to 10 kb can be easily amplified following general PCR protocol (still needs to prolong extension time). Targets of 10-15 kb may also be amplified, with some optimizations. The enzyme mixture is provided with an optimized buffer that improves enzyme fidelity and amplification of difficult templates. PCR products generated using HiFiTaq Polymerase contain dA overhangs at the 3´ end, and can be used directly for downstream TA cloning.
Higher-fidelity PCR of < 10 kb fragment
High-throughput PCR
Site-directed Mutagenesis
High-throughput PCR
Site-directed Mutagenesis
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|
10232-001 |
10232-005 |
|
HiFiTaq DNA Polymerase (5 U/μl) |
100 μl |
5 × 100 μl |
|
10× HiFiTaq PCR Buffer |
1 ml |
5 × 1 ml |
@ -20°C for 1 year. Avoid repeated freeze and thaw cycles.
• 6 times higher fidelity than Taq DNA Polymerase
• Amplification of PCR products up to 10 kb
• Great sensitivity with high yield
• Amplification of PCR products up to 10 kb
• Great sensitivity with high yield